lung carcinoma tissue microarray  (Johns Hopkins HealthCare)

Journal: Nature Communications

Article Title: Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression

doi: 10.1038/s41467-024-49199-9

Figure Lengend Snippet: a Representative immunofluorescence (IF) images and quantitative analyzes of the indicated markers in patient-derived lung tissues from non-smokers ( n = 3/group, 5 fields/slide [ n = 15/group]) and smokers ( n = 5/group, 6 fields/slide [ n = 30/group]). Scale bar: 100 μm. b Representative IF staining images of the indicated markers using a tissue microarray (TMA) ( n = 21 for the TNM N0 group; n = 14 for the TNM N1/2 group). Scale bars: 20 μm. c , left Correlation analysis for the Pearson correlation coefficient between IGF2 and GLUT1 levels in macrophages ( n = 35/group). c , right Correlation analysis for the Spearman rank correlation coefficient between nuclear pIGF-1R/IR (Y1131 for IGF-1R, Y1146 for IR) and PD-L1 in tumor cells ( n = 35/group). d Pie charts showing the levels of indicated markers in tumor tissues from patients with or without lymph node metastasis (N0 or N1/2, respectively; n = 21 for the TNM N0 group; n = 14 for the TNM N1/2 group). e Association of the expression level of the indicated markers with cancer stage (left, n = 16 for the stage I group; n = 14 for the stage II group; n = 5 for the stage III group) and tumor grade (right, n = 5 for the grade 1–2 group; n = 20 for the grade 2-3 group; n = 8 for the grade 3 group). f Analysis of a publicly available dataset (GSE30219) to determine the association of GLUT1 or GLUT3 levels with overall and disease-free survival (OS [ n = 136/group] and DFS [ n = 129/group], respectively) in patients with NSCLC. The data are presented as the mean ± SD. p -values were determined by using a two-tailed Student’s t -test ( a , b ), two-tailed Mann-Whitney test ( a ), one-way ANOVA with Tukey’s post-hoc test ( e ), Kruskal–Wallis test with Dunn’s post-hoc test ( e ), or a log-rank test ( f ). Source data are provided as a Source Data file.

Article Snippet: A human lung carcinoma tissue microarray (TMA) was purchased from US Biomax (Rockville, MD, USA; cat. no. LC821).

Techniques: Immunofluorescence, Derivative Assay, Staining, Microarray, Expressing, Two Tailed Test, MANN-WHITNEY

Journal: Theranostics

Article Title: Suppression of metastasis through inhibition of chitinase 3-like 1 expression by miR-125a-3p-mediated up-regulation of USF1

doi: 10.7150/thno.26467

Figure Lengend Snippet: Effect of USF1 on Chi3L1 expression, melanoma metastasis, and cancer cell growth and migration. (A) Immunohistochemistry (IHC) analysis to detect USF1-positive cells in human lung cancer tissue microarray (blue denotes nuclei; brown denotes USF1). Lung cancer tissue array, including TNM, clinical stage, and pathology grade; 75 cases/150 cores. Quantification of USF1 gene expression was measured and analyzed using ImageJ software. *, p <0.05. (B) IHC analysis to detect Chi3L1-positive cells in the human lung cancer tissue microarray (blue denotes nuclei; brown denotes Chi3L1). Lung cancer tissue array, including TNM, clinical stage, and pathology grade; 12 cases/24 cores. *, p <0.05; **, p <0.01. (C) Relative expressions of Chi3L1 mRNA levels by siUSF1 treatment compared to siControl. *, p <0.05. (D) Chi3L1 and USF1 protein expressions following siUSF1 treatment compared to siControl in A549 and H460 cells. (E) Trans-well migration assay of A549 and H460 cells treated with siUSF1. *, p <0.05. (F) Cell proliferation was measured by MTT assay of A549 and H460 cells treated with siUSF1. *, p <0.05. (G) Metastases on the surface of the lung in the B16F10 mouse melanoma-injected group (n=5). Black colony is B16F10 melanoma colonization on the surface of lung tissues of five grains of mice per group. (H) Count of total nodules in each lung tissue. *, p <0.05. (I) Image of B16F10 formation in lung tissues by hematoxylin and eosin. (J) IHC analysis to detect cells positive for Chi3L1 and USF1 in the lung tumor tissues of normal and USF1 KD mice (blue denotes nuclei; brown denotes Chi3L1). (K) Expressions of Chi3L1, PCNA, MMP-9, MMP-13, and VEGF proteins in the lung tumor tissues of normal and USF1 KD mice. Quantification of protein expression was measured and analyzed using ImageJ software. (L) Relative expressions of Chi3L1 mRNA by siUSF1 treatment compared to siControl. *, p <0.05; **, p <0.01. All in vitro experiments were prepared in duplicate and repeated three times. Values below the Western blot indicate densitometry analysis using ImageJ software.

Article Snippet: The human lung cancer tissue microarray, containing lung tumors from 140 patients and 10 samples of normal tissue, and the lung carcinoma tissue microarray, containing lung tumors from 12 patients and 12 samples of normal tissue, were purchased from US Biomax (Rockville, MD, USA).

Techniques: Expressing, Migration, Immunohistochemistry, Microarray, Software, MTT Assay, Injection, In Vitro, Western Blot

Journal: BMC Cancer

Article Title: RCC2 over-expression in tumor cells alters apoptosis and drug sensitivity by regulating Rac1 activation

doi: 10.1186/s12885-017-3908-y

Figure Lengend Snippet: RCC2 was over-expressed in lung cancers and ovarian cancers. a IHC of HeLa cells was performed with anti-RCC2 antibody (D14F3, cell signaling). RCC2 signals were seen in nucleus and midbody of a telophase cell as expected. Cytoplasmic RCC2 was also observed. b RCC2 expression in a lung cancer tissue microarray was evaluated by IHC. RCC2 were not seen in normal lung (left) but highly expressed in lung cancer (10×, scale bar: 100 μm). In addition to nuclei, RCC2 signals were also seen in cytoplasm in some lung cancers ( c ). d RCC2 expression in an ovarian cancer tissue microarray was evaluated by IHC. Normal ovaries expressed none or weak RCC2 (−~+) (left). Increased RCC2 expression was seen in majority of ovarian cancers

Article Snippet: Lung carcinoma progression tissue microarray (LC2083; Biomax; Rockville, MD, USA) and ovarian cancer and normal tissue high density tissue microarray (OV208; Biomax) were de-paraffinized in xylene, antigen-retrieved by heating in 0.01 M sodium citrate buffer (pH 6.0) at 95 °C for 10 min, blocked in 10% normal goat serum for 30 min and incubated with an anti-RCC2 antibody (D14F3; Cell Signaling, Danvers, MA, USA) overnight at 4 °C.

Techniques: Expressing, Microarray